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Role of TFIIS factors in the epigenetic control of genome rearrangements in Paramecium

Name: 
Mireille Bétermier
Team: 
Mireille Bétermier - Gif-sur-Yvette
Host: 
Jacek Nowak - Warsaw
Date: 
Tuesday, April 22, 2014 to Saturday, April 26, 2014
Report: 

Our collaboration with J. Nowak, K. Maliszewska and J. Gruchota (R. Gromadka's lab at the Institute of Biochemistry and Biophysics, Warsaw) has been going on since 2008. This work was initiated thanks to the support of the CNRS Program for International Cooperative Science (PICS), within the framework of the International consortium on “Paramecium Genome Dynamics and Evolution” and the COST network.

We have been studying the functional role of a development-specific paralog of transcription factor TFIIS on the synthesis of non-coding transcripts in the developing new somatic nucleus of Paramecium. Our data show that TFIIS4-dependent non-coding transcription originates from the non-rearranged version of the genome that is amplified just before programmed DNA elimination starts. This transcription is required for the correct elimination of a subset of germline sequences, which we have characterized.

The STSM allowed us to significantly advance our interpretation of the results and writing of the manuscript so that it can be shown to co-authors before submission. This involved intense and productive discussions with J. Nowak, corresponding author of the manuscript.

 

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