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Paramecium genetics

Team leader: 
Ewa Przybos
Team members: 
Malgorzata Prajer
Sebastian Tarcz
Natalia Sawka
Marta Surmacz
Institute or University: 
Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
Slawkowska 17
Krakow
31-016
Poland
Research interests: 

Faunistics, speciation of the Paramecium aurelia species complex, genetic differentiation of taxonomic species of Paramecium genus, molecular phylogeny, molecular genetics and functional analysis of the Paramecium genome.All studies are carried out using living strains belonging to our huge strain collection  (more than 400 strains) composed of P. aurelia spp. and other Paramecium species from different geographical regions of the world. 

Keywords: 
Paramecium, Paramecium aurelia species complex, molecular markers, genetic polymorphism, molecular phylogeny, mating types.
Techniques: 
Species differentiation by classical and molecular method, DNA sequencing, bioinformatic methods, RNA-interference, microinjection, cell biology techniques.
Publications: 

 

  • Przyboś E., Tarcz S., Prajer M., Surmacz M., Rautian M., Sawka N. 2012. Does high intraspecific variability of two genome fragments indicate a recent speciation process of Paramecium dodecaurelia (P. aurelia species complex, Ciliophora, Protozoa)? Systematics and Biodiversity 10: 289-304.
  • Arnaiz O., Mathy N., Baudry C., Malinsky S., Aury J.M., Denby Wilkes C., Garnier O., Labadie K., Lauderdale B.E., Le Mouël A., Marmignon A., Nowacki M., Poulain J., Prajer M., Wincker P., Meyer E., Duharcourt S., Duret L., Betermier M., Sperling L. 2012. The Paramecium germline genome provides a niche for intragenic parasitic DNA: Origin and evolution of internal eliminated sequences. PLoS Genet. 8(10): e1002984, doi:10.1371/journal.pgen.1002984
  • Greczek-Stachura M., Potekhin A., Przyboś E., Rautian M., Skoblo I., Tarcz S. 2012. Identification of Paramecium bursaria syngens through molecular markers – comparative analysis of three loci in the nuclear and mitochondria DNA. Protist 163: 671-685.
  • Przyboś E., Tarcz S., Potekhin A., Rautian M., Prajer M., 2012. A two-locus molecular characterization of  Paramecium calkins. Protist 164: 263-273.
  • Tarcz S., Potekhin A., Rautian M., Przyboś E. 2012. Variation in ribosomal and mitochondrial DNA sequences demonstrates the existence of intraspecific groups in Paramecium multimicronucleatum (Ciliophora, Oligohymenophorea). Mol. Phylogenet. Evol. 63: 500-509.
  • Przyboś E., Tarcz S., Greczek-Stachura M., Surmacz M. 2011. Polymorphism of Paramecium pentaurelia (Ciliophora, Oligohymenophorea) strains revealed by rDNA and mt DNA sequences. European Journal of Protistology 47: 138-143.
  • Haentzsch M., Bernhard D., Berendonk T. U., Przyboś E., Schegel M. 2011. Application of a multiplex PCR with specific PCR primers for the detection of the genus Paramecium and the Paramecium aurelia complex. Acta Protozool. 50: 235-238.
  • Przyboś E.,  Tarcz S., Greczek-Stachura M., Surmacz M. 2011. Seasonal and spatial variability of species occurence of the Paramecium aurelia complex in a single natural pond (Protista, Cilophora). Hydrobiologica 663: 233-244. 
  • Przyboś E., Barth D., Berendonk T. U. 2010. Paramecium sexaurelia – intra-specific polymorphism and relationship with other P. aurelia spp. revealed by cytochrome b sequence data. Folia biologica (Kraków) 58: 55-60.
  • Greczek-Stachura M., Tarcz S., Przyboś E. 2010. Intra-specific differentiation of Paramecium bursaria by molecular studies. Folia biologica (Kraków) 58: 35-45.
  • Przyboś E., Surmacz M. 2010. New, world-wide data on the distribution of species of the Paramecium aurelia complex (Ciliophora, Protozoa). Folia biologica (Kraków) 58: 185-188.
  • Nekrasova I., Przyboś E., Rautian M. S., Potekhin A. A. 2010. Electrophoretic karyotype polymorphism of sibling species of the Paramecium aurelia complex. Journal of  Eukaryotic Microbiology  57: 1-14. 
  • Catania F., Wurmser F., Potekhin A. A., Przyboś E. , Lynch M. 2009. Genetic diversity in the Paramecium aurelia species complex. Molecular  Biology and Evolution  26: 421-431.

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